Create an image stack (3-D matrix)

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Jack on 4 Jun 2013
Commented: r r on 20 Nov 2021
Hello. I'm importing a series of images from my working directory using the following code:
for i=139:141
% {} = cell array
images{i} = imread(sprintf('IMG_%04d.JPG',i));
I{i} = rgb2gray(images{i});
I have a large number of images, but here I'm importing just a sample. I'm converting each color image to grayscale. I must also create an image stack, a 3D matrix of grayscale images. I've been browsing the web for an answer, but I'm having difficulty understanding the process. size(I) yields '1 141', and I'm wondering how I can create a 3-D matrix from this. Your help would be greatly appreciated. Thank you!
  1 Comment
joseph robinson
joseph robinson on 19 Apr 2017
Adding to the feedback of others, your code would be more efficient without looping, e.g.,
idx = 139:141;
# image file paths
ipaths =cellfun(@(x) sprintf('IMG_%04d.JPG',x),idx,'uni',false);
# read images into cell array
I = cellfun(@imread, ipaths,'uni',false);
# Convert to gray-scale
Igray = cellfun(@rgb2gray,I,'uni',false);
# Concatenate 3 gray-scale images into single 3D matrix
myImage = cat(3,Igray{:});
Hope this helps!

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Accepted Answer

Kye Taylor
Kye Taylor on 4 Jun 2013
Edited: Kye Taylor on 4 Jun 2013
Execute this command after your code above to get the three gray-scale images you create into one 3D matrix of grayscale images;
myImage = cat(3,I{139:141});

More Answers (4)

Image Analyst
Image Analyst on 4 Jun 2013
Edited: Image Analyst on 4 Jun 2013
The (badly-named) variable I is a one dimensional array of cells. Inside each cell it a 2D array. So, in effect, this is a 3D array though you can't reference it like I(row, column, slice). If that is what you need to do then you need to do
I = []; % Before the loop
Then in the loop:
I = cat(3, I, images{i}); % Tack on slice i into a new cell.
In the meantime, please read the FAQ on cells to get a better idea of how they work:
Image Analyst
Image Analyst on 31 Jul 2021
@Jay Kandukuri, allocate an array of 80 slices, then loop and read in the files and load them in to the array you just allocated.
dcm3d = zeros(rows, columns, 80, 'uint8');
% Then in a loop, load them in
for slice = 1 : 80
thisImage = dcmread(filename); % filename must change on every iteration obviously.
dcm3d(:, :, slice) = thisImage;

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hf fh
hf fh on 21 May 2018
Edited: Walter Roberson on 21 May 2018
I have a question
I used the same method but I want to extract it in size format volume like figure 3D
how it would be???
clear all
folder = '/Users/mac/Desktop/fiel2/data';
D = '/Users/mac/Desktop/fiel2/data';
for i=1:8
% {} = cell array
images{i} = imread(sprintf('%d.tif',i));
I{i} = rgb2gray(images{i});
I = cat(3, I, images{i}); % Tack on slice i into a new cell.
Image Analyst
Image Analyst on 15 Apr 2020
This works for me:
folder = pwd; % 'C:\Users\Suraj\Desktop\images\Results';
% for k = 109 : 433
% fullFileName = fullfile(folder, sprintf('000%d.jpg', k));
for k = 1 : 10
fullFileName = fullfile(folder, sprintf('%d.jpg', k));
if ~isfile(fullFileName)
fprintf('File #%d not found : %s\n', k, fullFileName);
fprintf('Storing image file #%d : %s\n', k, fullFileName);
% Store the original image, regardless of whether it's RGB or gray scale.
originalImages{k} = imread(fullFileName);
% Store the gray scale version of the image. Convert (if necessary) from RGB to gray scale.
if ndims(originalImages{k}) > 2
grayScaleImages{k} = rgb2gray(originalImages{k});
grayScaleImages{k} = originalImages{k};
Change the comments to have it use your images in your folder, and in the range 109 to 433. Better yet, see if you can just use dir() to get a list of only those images that definitely exist.

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hf fh
hf fh on 21 May 2018
Please help me .. I searched a lot for convert 2Dimages into 3D but I still have the same problem !!! ''I can not get the images in volume in three dimensions" I have a search in 20 image dimensions [773x896]pixel, I want to convert it to 3D-three dimensions in volume form to study the depth, I following steps this: First:
Must be converted to a size of [300 * 300] pixels Second:
Converted to stack Third:
Converted to format==> 3D
like :
folder = '/Users/mac/Desktop/fiel2/data/';
for i=1:20
images{i} = imread( fullfile(folder, sprintf('%d.tif',i) ));
Q{i}=imresize(images{i} ,[300 , 300]);
I = cat(3, Q{i});
Thank you for help me ...
Walter Roberson
Walter Roberson on 16 Aug 2021
For your situation, the cat(3) that Image Analyst showed is correct.
Your difficulty is that you cannot use imshow() to display volumes. To display volumes, you need to use one of the following:
  • montage()
  • implay()
  • volumeViewer()
  • vol3d from the FIle Exchange
  • videofig from the File Exchange

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hf fh
hf fh on 21 May 2018
Thank you for answering me... I'm a beginner in using MATLAB and I just want to convert 20 images from 2D-two to 3D-three dimensions only. The aim is to convert from 20 images to an only volume 3D Look for of a method to apply the images.
I following steps like this:
First: Must be converted to a size of [300 * 300] pixels
Second: Converted to stack==>2D[300*300*20]
Third: Converted to format==> 3D volume
please help me What do I think about is true ??? and if you have a proposal please help me ..
  1 Comment
r r
r r on 20 Nov 2021
I have 30 images-2D and I want to convert it into a package(stack) and then convert it to 3D-volume, I use several methods but didn't get any result please help me?!!
clear all
folder = '/Users/Desktop/Slice3D/';
for k=1:30
images{k} = imread( fullfile(folder, sprintf('%d.jpg',k) ));
Q{k}=imresize(images{k} );
I = cat(3, Q{k});
V =squeeze(I);
image3d = zeros(512,512, 30, 'uint8');
for k = 1 : 30
% Read one image.
thisImage = imread( fullfile(folder, sprintf('%d.jpg', k) ));
% Store this image in the k'th slice of the 3-D image array.
%image3d(:, :, k) =imresize(thisImage);
image4d(:, :, :, k) = imresize(thisImage);
I = cat(3,image3d(:, :, k));
M =squeeze(I);

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