How to counting RBC size and number

i write this code but i cant get number of rbc and this is my input image

clear all;

close all;

clc;

count=0;

RC_Im = imread('red_cells.png');

RC_Im = rgb2gray(RC_Im);

level=graythresh(RC_Im);

RC_Im_BW = im2bw(RC_Im,level);

figure;

imshow(RC_Im_BW,[])

[x,z]=size(RC_Im_BW);

for R=0:1:60

SE = strel('disk',R);

afteropen=imopen(RC_Im_BW,SE);

if RC_Im_BW==1

count=count+1;

end

end

figure;

imshow(afteropen,[])

Answer 1

[Vinay]

Hi @Nora Hamad,

The binary image comparison 'RC_Im_BW==1' compares the entire image rather than individual objects.The 'bwlabel' can be used to label connected components in the binary image which counts the number of distinct objects.

%morphological operations
RC_Im_BW_cleaned = imopen(RC_Im_BW,strel('disk', 5));

% Label connected components
[labeledImage, numRBCs] = bwlabel(RC_Im_BW_cleaned);

% Print the count of RBCs
fprintf('Number of RBCs: %d\n', numRBCs);

Refer to the below documentation of 'bwlabel' for more details.

https://www.mathworks.com/help/images/ref/bwlabel.html