RGB to H&E channel transformation

how to Convert RGB image to H&E channel or color space?Codings needed..H&E channel transformation..

[Image Analyst]

I've never heard of the H&E color space. Do you have a website reference for this transformation? Why do you want to use that instead of the much more common HSV, HSI, HSL, or LAB color spaces?

[angelina]

I'm working in medical imaging project.I need to differentiate nucleus and others from this H&E stained image(RGB format).So i need to transform RGB image to H&E (Haematoxylin and eosin ).http://en.wikipedia.org/wiki/H%26E_stain.is it possible to do it in matlab for leukemia classification?

[Gang Luo]

Have you solved the problem? I am also trying to differentiate nucleus and others from this H&E stained image(RGB format).Maybe I need your help.

[Nancy]

how to identify a h and e image into RGB image

[Image Analyst]

@Nancy I would think you would know if you had an H&E image or not. Since you asked, I'm assuming that you don't know and you're looking for some automated way to tell you if some arbitrary image is an H&E image or not. Is that right? I'd suggest you create a deep learning network. Take a bunch of your known H&E images, and a bunch or other arbitrary images, and train the network. Then with the trained model, run your unknown images through it. For starters, I'd look for demos on CNN transfer learning where you can train a classifier to detect H&E or non-H&E images.

Answer 1

[ImageProcNaive]

You can use this workflow:

1. Get RGB vector values for each of the stains using the approach mentioned in the "Determining New Vectors" section here: Determining New Vectors
2. Then update the values in this code and run to separate your H&E images: Color Deconvolution