Objects Segmentation in the images

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Dear All,

now i am analysing the primary particles from aggregates, it is too small, when i use watershed segmentation, which separate overlapp particels not so well, do u have any ideas to improve this problem?

after i use watershed, it shows as below

and i want to show after segmentation, not show like this RRB, but the segmentation lines in the original image.

this is watershed cold i use.

% Distance transform

DT=-bwdist(~DRemove6);

DT(~DRemove6)=-Inf;

% Watershed

W=watershed(DT);

imshow(label2rgb(W,'jet','w'))

title('Watershed Transform')

Thanks so much for your advance.

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mohd akmal masud on 18 Jun 2023

Open in MATLAB Online

you can use image segmenter apps.

Also, you can use avtive contour segmentation

clc;close all;imtool close all;clear;
G = imread('I2_09_4_2_nuclei.jpg');%read your image
imshow(G);
mask = zeros(size(G));
mask(25:end-25,25:end-25) = 1;
imshow(mask);shg;
imshow(G); shg; hold on;
b = bwboundaries(mask, 'noholes');
plot(b{1}(:,2),b{1}(:,1),'r','LineWidth',3);
hold off;
stepsize=10;
for k = 1:400/stepsize
    mask = activecontour(G, mask, stepsize);
  
    imshow(G); title(sprintf('Iteration %d',k*stepsize),'FontSize',16);
    b = bwboundaries(mask, 'noholes'); 
    hold on;
    for k=1:size(b)
        plot(b{k}(:,2),b{k}(:,1),'r','LineWidth',3);
    end
    hold off;
    drawnow;
end
imageSegmenter(G);