Two scripts for the analysis of platelet aggregation in microfluidic channels after whole blood perfusion. The first script, “Platelet_Coverage”, automatically corrects for channel misalignment, determines a threshold using the triangle method, creates a black and white image representing platelet coverage and finally determines the platelet coverage. The second script, “Clot_Properties”, uses the black and white image from the first script to determine the platelet aggregate size distribution.
Triange Thresholding is done using a script of dr. Panneton (MathWorks, #28047) which is based on the original work of Zack, Gregory W., William E. Rogers, and S. A. Latt. "Automatic measurement of sister chromatid exchange frequency." Journal of Histochemistry & Cytochemistry 25.7 (1977): 741-753.
Hugo Albers (2022). Automated Analysis of Platelet Aggregation in Microfluidics (https://www.mathworks.com/matlabcentral/fileexchange/73348-automated-analysis-of-platelet-aggregation-in-microfluidics), MATLAB Central File Exchange. Retrieved .
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Inspired by: Gray image thresholding using the Triangle Method
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