How to distinguish each fiber and analyze the properties of the fiber?
3 views (last 30 days)
I am currently trying to develop a code to analyze each fiber using the Image Processing Toolbox. The image I am working with is a microscopic image of the cross section of the fiber. I tried thresholding the image, but since the single fibers have shades of light, it is extremely difficult for Matlab to distinctly define the individual fibers. The background(empty space) is also bright so there is no real distinction between parts of the fiber and the background space. When I converted the colorimg to a grayscale then to a binaryimg, the binaryimg was not recognizing the each single fiber. I am just wondering if there is a more efficent approach to analyzing this img?
The other way I believe I can alleviate this problem is if I dye the fibers so there is a better distiction between the fiber and the background light.
Thanks in advance to who helps!
Image Analyst on 9 Sep 2013
Of course if you can get a background that is not in the black to gold/yellow spectrum you will get more contrast and be able to segment these better. For example, something in the greenish/bluish hue range (a hue diametrically opposite gold). In addition, is it possible to separate the fibers so that they aren't touching? It's always better to start with a good image than to try to fix up a bad one post-acquisition.