Tumor grading in GUI or ...?
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I have micro tumor images(9) and there are areas of dlbcl tumor (Nd DAB-stained and Nh H-stained) Proliferation index for this is a solution of the equation PIc=Nd/(Nh+Nd). I have to find PIc for all pictures knowing RGB intervals for Nh= (<45, 180>, <50, 185>, <160, 215>) and for Nd= (<40, 115>, <6,80>, <10,75>). How AM i supposed to do it? Please help!!
4 Comments
Image Analyst
on 22 Nov 2018
And the equations in my answer below don't do it? Please explain why not. And attach your image.
Answers (4)
Image Analyst
on 22 Nov 2018
Use the code in Jan's comment above, but modify it to get two binary images: matchNh and matchNd. Then I think you want the sum (count) and ratio
Nd = nnz(matchNd)
Nh = nnz(matchNh)
Plc = Nd / (Nh + Nd)
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Image Analyst
on 22 Nov 2018
Try this:
4 Comments
Image Analyst
on 23 Nov 2018
Look at the gamuts - they're essentially a 3-D histogram where there is one point for every (r,g,b) triplet (every pixel value/color).
Point 1: note how there are no well contained clusters - it's basically a continuum - so the dividing plane will essentially be sort of arbitrary. You could almost put it wherever you want but at least kmeans has some sort of logic behind where it splits the gamut into the 2 colors.
Point 2: thresholding essentially divides up the gamut by slicing the color could with planes aligned with the axes, and you can see that there are no planes perpendicular to the R, G, and B axes that will do even a half way decent job of dividing the image up into different colors that are meaningfull. With HSV color space, your dividing surfaces are basically cones, sectors, and planes perpendicular to the V axis. By doing that, you can definitely carve out different regions because the hue, saturation, and brightness are different between the pink, purple, and white. The R, G, and B are also different, but you can see that planes perpendicular to the axes will not carve that cloud up into pink, purple, and white sub-clouds.
Aneta Chwala
on 27 Nov 2018
3 Comments
Image Analyst
on 27 Nov 2018
Yes, you may.
If it's unrelated to your first one way at the top, then create a new thread.
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