Preprocessing

Manage NGS data with single- and paired-end reads, filter and trim reads, and display quality statistics

Preprocess sequence read data using various functions. Filter reads based on the quality of sequencing data and trim termini of sequences. Perform barcode matching (demultiplexing) to group samples based on their barcodes. Split merged paired-end sequence data into two separate files. Calculate quality statistics and visualize such information using quality control plots.

Functions

seqfilterFilter out sequences based on specified criterion
seqtrimTrim sequences based on specified criterion
seqsplitSplit sequences into separate files based on barcodes
seqsplitpeSplit merged paired-end sequences into separate files
seqqcplotCreate quality control plots for sequence and quality data